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Calibration is performed to make sure that PeriFlux Units around the world measure the same values. The instrument and the probes are calibrated to the measuring points 0 PU and 250 PU. The calibration should be checked minimum every month. Full calibration should be carried out when the values are out of the permitted range. Always check the calibration when a new probe is attached or before sending probes for sterilization.
It is especially important to calibrate if the measuring range is low. For example, when measuring on teeth, a displaced ZERO could be very misleading, as the range can be 0-10 PU.
Check calibration
Check calibration by measuring in the Motility Standard (steps 1-3 below) and on the Zeroing Disc. A full calibration should be performed if the obtained values are other than:
• 250 ±15 PU in Motility Standard
• 0 ± 1 PU on the Zeroing Disc
• Total Backscatter (TB) > 2V (if recorded)
Full calibration
1. Allow the instrument 20 minutes to warm up.
2. Shake the bottle containing the Motility Standard. Carefully fill the glass container with Motility Std.
Avoid bubbles. Place on a vibration free table/floor.
3. Place the probe tip 5 mm into the liquid. Tap cautiously to remove potential air bubbles at the tip.
4. Press the Calibration button on the instrument.
5. When finished, drain the Motility Standard back into the bottle (avoids evaporation) or secure the container lid tightly.
Verifying Zero
Check the zero level by placing the probe on the Zeroing Disc: PROBE 407 is placed in probe holder PH07 and attached to the Zeroing Disc with double adhesive strips. Flat probes, e.g. PROBE 457 and 404, are fastened directly on the Zeroing Disc with double adhesive strips. PROBE 418 is positioned in the small hole inside the largest cavity. Probes with tips > 12 mm are placed in a suitable hole.
Always avoid space between the probe tip and the Zeroing Disc!
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