Occlusion of the middle cerebral artery (MCA) in mice or rats is widely used as a model to study stroke. The occlusion is often produced by inserting filaments into specific arteries. One drawback with the model is that it is not always straightforward to confirm that an occlusion has occurred. Therefore, many researchers use the PeriFlux System 5000 as a quality control to ensure that a stroke has been induced before any further experiments are carried out. In principle, the PeriFlux System 5000 equipped with either PROBE 407 (rats) or PROBE 418 (mice) is used to continuously monitor the blood perfusion in the brain before and after occlusion. A 70% drop in blood perfusion is expected if a successful occlusion has been established.
To further investigate the induced stroke, our blood perfusion imager PeriCam PSI System is an interesting alternative. The PeriCam PSI System visualizes the spatial distribution of the cerebral blood flow in both hemispheres and allows you to observe any dynamic changes in real-time. The PIMSoft software used for data evaluation includes functions allowing you to quantify the extension of the stroke in mm 2 and to calculate the blood perfusion levels.
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