PeriCam PSI

PSI Z

PeriCam PSI System is a blood perfusion imager based on the laser speckle contrast analysis (LASCA) technology, also known as laser speckle contrast imaging (LSCI). It is a method that visualizes tissue blood perfusion in real-time. LASCA provides new means to study the microcirculation in ways that were not possible in the past. PeriCam PSI System combines dynamic response and high spatial resolution in one instrument, providing both real-time graphs and video recordings of the tissue being studied. To further enhance its usability, dedicated application software, PIMSoft, has been developed.

PeriCam PSI Z

PSI Z is the latest addition to the PeriCam PSI family. This instrument is built with a fast motorized zoom lens for optimal flexibility: zoom in for higher resolution and zoom out for a larger measurement area (up to 25 x 25 cm). This, combined with auto-focus capability, makes it a great member of our imaging family.

Some specifications:

Maximum image size: 25 x 25 cm (625 cm2)
Imaging camera pixels: 4.2 million (2048 x 2048)
Zoom range: 3x optical zoom with auto-focus
Max resolution at 20 x 20 mm area: 10 µm/pixel
Max area at 10 µm/pixel: 20 x 20 mm (400 mm2)
Image acquisition rate: Up to 96/113 images per second depending on frequency of background light
Advanced background removal: Continuous during measurement with automatic frequency detection and synchronization
Working distance: 10-32 cm

PeriCam PSI Z is an excellent tool for imaging of the cerebral blood flow (CBF) in mice and rats, e.g., for stroke models (MCAO) and cortical spreading depolarization (CSD). Other common applications are angiogenesis models such as the hindlimb ischemia (HLI) model.

Please note that PSI Z is intended for research purposes in USA and China. PSI Z is planned for launch in Europe in 2020.

System design

PeriCam PSI System uses an invisible near infra-red (NIR) laser (785 nm) for blood perfusion measurements. The beam is spread over the measurement area by a diffuser, creating a speckle pattern. The size of the illuminated area depends on the distance between the laser aperture in the head and the measurement object.
The speckle pattern in the illuminated area is monitored using a 2048 x 2048-pixel camera that can take images at a speed of more than 100 frames per second. Blood perfusion is calculated by analyzing the variations in the speckle pattern. For more information about the measurement technique, see the page about LASCA.
To facilitate the positioning of the imager relative to the subject, visible red lasers (650 nm) are used to indicate the measurement position. To avoid interference with measurements, the indicator lasers are switched off during recording. Both the measurement laser and the indicator lasers are safe to use without eye protection.
A separate color camera is used for documentation. The capture rate can be set at different speeds during a recording, up to one photo per second. The color photos provide an extra help when analyzing the perfusion data, assuring that the subject has not moved during the recording and for understanding the obtained blood perfusion images.

Dedicated software: all-in-one data analysis

The instrument comes with a dedicated software package, PIMSoft. A complete, all-in-one solution for setup, acquisition, analysis and reporting.
Blood perfusion is presented both as color-coded images and in graphic form, enhancing the possibilities for understanding and analyzing the microcirculatory data. Specific ROIs can be defined before, during or after recording. In addition, ROIs may be implemented during sections of a run. Time periods of interest (TOIs) allow for evaluation of blood perfusion during specific time periods. Event markers with comments may be added at any time.
Automatically calculated values include mean, percentage change, number of measurement points, standard deviation and more. Selected data is conveniently exported as PDF reports or in XML format for further processing.

Please note that PSI Z is intended for research purposes in USA and China. PSI Z is planned for launch in Europe in 2020.

All in one data analysis
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