{"id":846,"date":"2024-12-19T13:10:27","date_gmt":"2024-12-19T13:10:27","guid":{"rendered":"http:\/\/www.perimed-instruments.com\/us\/?post_type=application&p=846"},"modified":"2025-01-16T11:48:25","modified_gmt":"2025-01-16T11:48:25","slug":"hindlimb-ischemia","status":"publish","type":"application","link":"https:\/\/www.perimed-instruments.com\/us\/applications\/preclinical-research\/hindlimb-ischemia\/","title":{"rendered":"Hindlimb ischemia"},"content":{"rendered":"
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Home<\/a><\/span> \/ Applications<\/a><\/span> \/ Preclinical research<\/a><\/span> \/ Hindlimb ischemia<\/span><\/span><\/div>\n\n\n

Hindlimb ischemia<\/h1>\n\n\n\n

Murine hindlimb ischemia is an important model for studying peripheral artery disease (PAD) and developing new therapies for it. It works by surgically inducing acute limb ischemia to assess physiological reactions such as vascular regeneration, angiogenesis, and arteriogenesis. <\/p>\n\n\n\n

The model can be used to evaluate the pro-angiogenic effects of therapeutic agents [1]<\/a><\/sup> or natural recovery in healthy [2]<\/a><\/sup> compared with diseased (diabetic) [3][4][5]<\/a><\/sup> young vs. aged [6]<\/a><\/sup>, and wild-type contra knockout animals [7][8][9]<\/a><\/sup>.<\/p>\n\n\n

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Data collection challenges<\/h2>\n\n\n\n

Data variability in this model can be created by the surgical method as well as the genetic background, age, and sex of the animal. For measurement tools, consistent placement of the imaging areas over time is a major challenge.<\/p>\n\n\n\n

To address this challenge, PeriCam PSI includes support for regions of interest (ROIs)<\/strong> and time periods of interest (TOIs)<\/strong>. Once ROIs are created, they can be saved and reused for subsequent measurements. To compensate for animal or camera movement, you can adjust the position of an ROI throughout a recording \u2014 providing a powerful tool for assessing perfusion changes over time.<\/p>\n\n\n <\/div>\n <\/section>\n\n\n

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PeriCam PSI in practice<\/h3>\n\n\n\n

Here, you can see how PeriCam PSI visualizes blood perfusion in a mouse before surgery and during the subsequent 28 days of recovery.<\/p>\n\n\n\n

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Image courtesy of Dr. Mario Images provided by Dr. Yi Tan, Pediatric Research Institute, University of Louisville School of Medicine[10]<\/a><\/sup><\/em>.<\/p>\n\n\n\n

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PeriCam PSI HR<\/h3>\n\n\n
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PeriCam PSI leverages laser speckle contrast imaging (LSCI) to assess blood perfusion in tissue at the microcirculation level with detail and precision. PeriCam PSI HR (high resolution) works from a fixed distance and is specifically equipped to measure small animals such as rats and mice.<\/p>\n\n\n

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