{"id":853,"date":"2024-12-19T13:31:43","date_gmt":"2024-12-19T13:31:43","guid":{"rendered":"http:\/\/www.perimed-instruments.com\/us\/?post_type=application&p=853"},"modified":"2025-01-15T13:27:22","modified_gmt":"2025-01-15T13:27:22","slug":"cam-assay","status":"publish","type":"application","link":"https:\/\/www.perimed-instruments.com\/us\/applications\/preclinical-research\/cam-assay\/","title":{"rendered":"CAM assay"},"content":{"rendered":"
\n \n
<\/div>\n
<\/div>\n
<\/div>\n \n\n
\n \n\n
\n
\n
Home<\/a><\/span> \/ Applications<\/a><\/span> \/ Preclinical research<\/a><\/span> \/ CAM assay<\/span><\/span><\/div>\n\n\n

Chorioallantoic membrane \u2014 CAM assay<\/h1>\n\n\n\n

The CAM assay is a versatile animal model for studying tumor formation, angiogenesis, and metastasis. It is highly reproducible, cost-effective, and has a natural immunodeficiency making it suitable for cell transplantation. The model relies on the creation of a window in the shell of a fertilized chicken egg, which enables researchers to observe the embryo and the formation of blood vessels.<\/p>\n\n\n

\n <\/div>\n\n\n\n
\n
Learn more<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n
\n \"\"\n\n <\/div>\n <\/div>\n<\/div>\n\n\n <\/div>\n <\/section>\n\n\n
\n \n\n
\n \n\n

Measuring with PeriCAM PSI HR<\/h2>\n\n\n
\n \"Background\n \"\"\n\n <\/div>\n \n\n
\n <\/div>\n\n\n\n

PeriCam PSI can be used to measure the (change in) blood perfusion in the angiogenesis assay and detect differences in the efficacy of various pro-angiogenic compounds.<\/p>\n\n\n\n

The data enables you to monitor the formation of functional intratumoral blood vessels[1]<\/a><\/sup> and validate the effectiveness of anti-angiogenic agents for cancer treatment.<\/p>\n\n\n

\n <\/div>\n\n\n <\/div>\n <\/section>\n\n\n
\n \n\n
\n \n\n

Overcoming data challenges<\/h3>\n\n\n\n

Researchers often use the CAM assay to study angiogenesis, which requires detailed measurements of blood vessel growth, density, and branching patterns. Quantifying these changes using image-based methods can be challenging as varying light conditions and magnification can make it difficult to standardize measurements and achieve reproducible data. PeriCam PSI HR includes automated background compensation and features for setting measurement distance and magnification.<\/p>\n\n\n\n

High-resolution imaging is required to capture subtle vascular changes on the CAM. Achieving consistently high image quality without introducing artifacts can be difficult. The translucent nature of the CAM and its sensitivity to light exposure can impact image clarity. PeriCam PSI HR records at a frequency of up to 100 images per second with a max resolution of 10 \u03bcm\/pixel. Leveraging LSCI. PeriCam PSI HR enables researchers to capture dynamic changes in microcirculation and tissue perfusion with precision.<\/p>\n\n\n <\/div>\n <\/section>\n\n\n

\n \n\n
\n \n\n

PeriCam PSI HR<\/h3>\n\n\n\n

PeriCam PSI leverages laser speckle contrast imaging (LSCI) to assess blood perfusion in tissue at the microcirculation level with detail and precision. PeriCam PSI HR (high resolution) works from a fixed distance and is specifically equipped to measure small animals such as rats and mice.<\/p>\n\n\n

\n <\/div>\n\n\n\n
\n
\n